Targeted sequencing of M. tuberculosis to examine BDQ treatment failu... - SRA

SRX14373214: Targeted sequencing of M. tuberculosis to examine BDQ treatment failure
1 ILLUMINA (Illumina MiSeq) run: 69,130 spots, 41.6M bases, 25.6Mb downloads

Design: Known BDQ-resistance associated regions were analysed, namely Rv0678, atpE and pepQ using multiple amplicons to sequence each target. Samples were pooled and sequenced on an Illumina MiSeq 2 x 300-bp sequencing run with V3 chemistry, or a NextSeq 500 2x150 kit,J5 with a targeted coverage of 30,000 reads per amplicon. At least 20% of each sequencing run was filled with PhiX control, or other amplicon libraries whose composition varied from that of this sample set to ensure base diversity. J3:J156Known BDQ-resistance associated regions were analysed, namely Rv0678, atpE and pepQ using multiple amplicons to sequence each target (Table 1 and Supplementary Document 1, Table S1). Samples were pooled and sequenced on an Illumina MiSeq 2 x 300-bp sequencing run with V3 chemistry, with a targeted coverage of 30,000 reads per amplicon. At least 20% of each sequencing run was filled with PhiX control, or other amplicon libraries whose composition varied from that of this s+J19ample set to ensure base diversity. J4Known BDQ-resistance associated regions were analysed, namely Rv0678, atpE and pepQ using multiple amplicons to sequence each target (Table 1 and Supplementary Document 1, Table S1). Samples were pooled and sequenced on an Illumina MiSeq 2 x 300-bp sequencing run with V3 chemistry, with a targeted coverage of 30,000 reads per amplicon. At least 20% of each sequencing run was filled with PhiX control, or other amplicon libraries whose composition varied from that of this sample set to ensure base diversity.

Submitted by: TGen North

Study: Mycobacterium tuberculosis genome sequencing

PRJNA767896 • SRP339739 • All experiments • All runs

show Abstracthide Abstract

Sequencing of Mycobacterium tuberculosis to examine causes of Bedaquiline treatment failure

Sample:

SAMN26442572 • SRS12185986 • All experiments • All runs

Organism: Mycobacterium tuberculosis

Library:

Name: B90_AMP.0

Instrument: Illumina MiSeq

Strategy: AMPLICON